CRISPR-Cas13a mediated nanosystem for attomolar detection of canine parvovirus type 2
نویسندگان
چکیده
منابع مشابه
Nucleic acid detection with CRISPR-Cas13a/C2c2.
Rapid, inexpensive, and sensitive nucleic acid detection may aid point-of-care pathogen detection, genotyping, and disease monitoring. The RNA-guided, RNA-targeting clustered regularly interspaced short palindromic repeats (CRISPR) effector Cas13a (previously known as C2c2) exhibits a "collateral effect" of promiscuous ribonuclease activity upon target recognition. We combine the collateral eff...
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BACKGROUND: Canine Parvovirus (CPV) is one of the major causes of fatal gastroenteritis in young dogs. After emergence of CPV-2, two new variants were reported and named CPV-2a and CPV-2b. In 2001 the newest variant was reported and named CPV-2c. The difference between CPV strains is related to amino acid changes in viral capsid protein (VP2). OBJECTIVES: The aim of the present study was geneti...
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Two male dogs at the age of 3 and 5 months of Doberman pinscher and German shepherd breeds were referred to the Veterinary Hospital of Ahvaz University with clinical signs of depression, vomiting, haemorrhagic diarrhoea, profound dehydration, fever and anorexia. The affected dogs had not the history of vaccination. Concurrent infection of canine parvovirus and coronavirus was detected in dogs b...
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ژورنال
عنوان ژورنال: Chinese Chemical Letters
سال: 2019
ISSN: 1001-8417
DOI: 10.1016/j.cclet.2019.10.032